El-Kholy MM, Ayoub MS, El-Malahy HM. The Effect of Wharton's Jelly Mesenchymal Stem Cells on a Squamous Cell Carcinoma Cell Line. Archives in Cancer Research. 2016.Abstract

<p><strong style="line-height: 1.5;">Title:&nbsp;</strong><span style="line-height: 1.5;">This experiment explores the effect of effect of Wharton’s jelly mesenchymal stem cells (WJMSCs)-conditioned medium on the HEP-2 cell line.</span></p>
<p><strong>Background:</strong>&nbsp;Wharton’s jelly mesenchymal stem cells may be more useful for cancer therapy than other adult stem cells since they are easily prepared in relatively large quantities from the umbilical cord after delivery. This study aims to determine the anticancer effect of WJMSC-conditioned medium on a HEP-2 cell line after 24, 48, and 72 hours using flow cytometry and real-time polymerase chain reaction.</p>
<p><span><strong>Methods and findings</strong>:&nbsp;</span>A HEP-2 cell line and WJMSCs were propagated. The cultured medium of WJMSCs was collected and used to substitute the cultured medium of the HEP-2 cell line. The HEP-2 cells were assessed after 24, 48, and 72 hours. We found that WJMSC-conditioned medium caused apoptosis in the HEP-2 cell line. The percentage of apoptosis was directly proportional to time. Expression of p53 was increased after 24 and 48 hours, but after 72 hours its expression decreased. The expression of Bcl-2 also decreased over time.</p>
<p><strong>Conclusions:</strong>&nbsp;It could be concluded that WJMSC-conditioned medium could be used as an apoptotic agent against malignant cells.</p>

Elsayed HH. The Sixth Complication of Diabetes. In: The international Conference of National Institute of Diabetes & Endocrinology . The international Conference of National Institute of Diabetes & Endocrinology . Grand Nile tower Cairo, Egypt; 2016. p. .